Secretome of adipose derived stem cells induced apoptosis in anaplastic thyroid carcinoma C-643 cells

Introduction: Previous studies have proven that secretory materials of mesenchymal stem cells (MSCs) (secretome) remedial properties. Objectives: The main goal this study was to evaluate the effects secretome adipose-derived MSCs on growth and apoptosis in anaplastic thyroid carcinoma (ATC) C-643 cells. Materials Methods: Initially, were exposed 25 50 μg/mL (ADSCs) for 24 48 hours, proliferation cytotoxicity cells, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test colony assay conducted. Acridine orange/ethidium bromide (AO/EB) staining conducted apoptosis. expression level apoptosis-associated genes determined by real-time polymerase chain reaction (PCR) technique. Results: Cell viability numbers groups significantly lower than control group. amount apoptosis-related (Bax/Bcl-2, P53, caspase-3 caspase-8) secretome-treated more Conclusion: data revealed ADSCs caused significant reduction cell induced